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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome C (CYC, Cytochrome C Somatic, CYCS, HCS, THC4)

Anti -Cytochrome C (CYC, Cytochrome C Somatic, CYCS, HCS, THC4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Cytochrome c is a requisite component of the mitochondrial respiratory chain and participates in the electron transfer between complex III and complex IV of the respiratory chain. Cytochrome c is a soluble protein that is localized in the mitochondrial intermembrane space and is loosely attached to the surface of the inner membrane. The protein is synthesized on cytoplasmic ribosomes as apocytochrome c and then translocated into the mitochondria via a unique pathway independent of the general protein translocation machinery, protease-sensitive components of the outer membrane, or a membrane potential across the inner membrane.
In response to a variety of apoptotic stimuli, cytochrome c is released from mitochondria into the cytosol. This release can be blocked by Bcl-2 and suggests that Bcl-2 may function by regulating cytochrome c release. Cytosolic cytochrome c is an essential component of the vertebrate apoptosome which is composed of cytochrome c, Apaf-1, and procaspase-9 (Apaf-3). Interaction of these proteins leads to the activation of caspase-9, which in turn activates other caspases (such as caspase-3) ultimately resulting in cell death. The current working hypothesis is that release of cytochrome c from mitochondria commits a cell to die either by a “rapid” apoptotic mechanism involving activation of the Apaf-1/caspase-9 cascade, or by a “slower” necrotic process involving the collapse of the electron transport chain.
Catalog #C9095-04B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot (denatured): 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa (human) and rat6 (rat) cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No7H8.2C12
HostMouse
SourceEquine
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenEquine Cytochrome c
SpecificityReacts with non-native (denatured) cytochrome c and does not recognize native Cytochrome c. Species Crossreactivity: Human, mouse, rabbit, rat, frog and Drosophila.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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