| Cytochrome c is located in the mitochondria of all aerobic cells and is involved in the electron transport system that functions in oxidative phosphorylation. It accepts electrons from cytochrome b and transfers them to cytochrome oxidase. In the process, the iron of the heme |
|
| group, which is identical to that of hemoglobin and myoglobin, shifts from the ferrous to the ferric state (1). Recent progress in studies on apoptosis has revealed that cytochrome c is a pro-apoptotic factor. It is released from its places on the outer surface of the inner mitochondrial |
|
| membrane at early steps of apoptosis and, combining with some cytosolic proteins, activates conversion of the latent apoptosis-promoting protease pro-caspase-9 to its active form. Cytochrome c release can be initiated by the pro-apoptotic protein Bax. This process is blocked by the anti-apoptotic proteins Bcl-2 and Bcl-xL (2). Also, differences in amino acid composition of cytochrome c have also been used as markers of biochemical evolution. |
|
| |
|
| Applications: |
|
| Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications have not been tested. |
|
| Catalog # | C9095-04F |
| Recommended Dilution | Western Blot: 1:250-500 |
| Immunohistochemistry: 1:100-250 |
| Immunocytochemistry: 1:50-100 |
| Immunoprecipitation: 1:50 |
| Optimal dilutions to be determined by researcher. |
| Storage and Stability | |
| May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 8k2 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium azide, 0.05% BSA and 40% Glycerol. |
| Purity | Supernatant |
|
| Immunogen | A synthetic peptide corresponding to residues near the N-terminus of human Cytochrome C |
| Specificity | Recognizes human Cytochrome C. Species Crossreactivity: mouse and rat. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
