| Cytochrome C is an electron transporting protein that resides within the inter-membrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event, which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis. |
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| Catalog # | C9095-04H |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 5ug/ml |
| Immunohistochemistry: 1:100 for 30min at RT |
| Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | HeLa cells, cardiac muscle |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG,k |
| Clone No | 2D17 |
| Host | Mouse |
| Source | Pigeon |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptides corresponding to aa1-80, 81-104 and 66-104 of pigeon cytochrome C. |
| Specificity | Recognizes pigeon cytochrome C. Species Crossreactivity: Human, mouse, rat, equine, canine, frog, Drosophila. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
