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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome C (CYCS, CYC)

Anti -Cytochrome C (CYCS, CYC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Cytochrome C is an electron transporting protein that resides within the inter-membrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event, which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis.
Catalog #C9095-04H
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 5ug/ml
Immunohistochemistry: 1:100 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cells, cardiac muscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG,k
Clone No2D17
HostMouse
SourcePigeon
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptides corresponding to aa1-80, 81-104 and 66-104 of pigeon cytochrome C.
SpecificityRecognizes pigeon cytochrome C. Species Crossreactivity: Human, mouse, rat, equine, canine, frog, Drosophila.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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