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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified B

Cytochrome C/Apaf-2 (apoptosis protease activating factor-2) is well known for its role in electron transport. During apoptosis (programmed cell death), Cytochrome C is released from the mitochondria into the cytosol where it binds to Apaf-1. Apaf-1 then activates caspase 9. Caspase 9 activation triggers other downstream caspase events in the proteolytic cascade. The mechanism by which Cytochrome C is released from the mitochondria and its function in Caspase 9 activation is still unclear.
Catalog #	C9095-09A
Applications
	Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution	Western Blot: 1ug/ml
	Cell extracts from 293T cells (2x10e6) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with C9095-09A at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system.
	Optimal dilutions to be determined by the researcher.
*Recommended Secondary Antibodies	I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
	I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	6D337
Host	Mouse
Source	Equine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.02% sodium azide.
Purity	~95% (SDS-PAGE)
Immunogen	Equine Cytochrome C
Specificity	Recognizes equine apo-Cytochrome C. Species Crossreactivity: human, mouse, rat, goat, sheep and monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.