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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome P450 (CYP2E1)

Anti -Cytochrome P450 (CYP2E1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Ethanol-inducible cytochrome P450 IIE1 (CYP2E1) is considered one of the major causes of oxidative stress in the liver following ethanol treatment. Radicals produced by this enzyme are thought to induce lipid peroxidation reactions and other damage to the cells and to be a major cause for ethanol-dependent liver toxicity. The importance of CYP2E1 lies in its ability to metabolize a wide range compounds such as organic solvents, acetaminophen, dimethylnitrosamine and aliphatic alcohols, which have relevant toxicological effects in humans. To date, over 75 substrates of CYP2E1 have been identified. CYP2E1 is mainly found in the liver although it has also been detected in a variety of other organs, including the brain, colon and lungs. In the liver it is localized to the centrilobular region, specifically the hepatocyte layers most proximal to the central vein. Cytochrome P450 is considered to be part of the mixed function oxidase (MFO) sytem along with NADPH-cytochrome P450 reductase.
Catalog #C9095-10
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenNative rat cytochrome P450
SpecificityRecognizes rat Cytochrome P450 at~50-55kD. Inhibits microsomal benzene metabolism. Species Crossreactivity: human, mouse, canine, monkey, guinea pig, rabbit, clam and equine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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