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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome P450 1A2

Anti -Cytochrome P450 1A2

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified B
Catalog #C9095-11M
ApplicationsSuitable for use in inhibition assays and Western Blotting.
Working DilutionA dilution of 1:2000 for Western blots will yield a positive result with 0.2ug of recombinant human cytochrome P450 IA2 using alkaline phosphatase-conjugated anti-goat IgG secondary antibody according to the manufacturer's instructions.
Inhibits approximately 80% of the ethoxyresorufin O-deethylase activity of human liver microsomes at a concentration of 10mg IgG/nmol P450.
Western Blot Recognizes a band (P450 1A2) in human liver microsomes.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration10mg/ml
FormSupplied as a liquid in 20mM Phosphate buffer, pH 8.0.
PurityPurified
ImmunogenRecombinant human P450 1A2 expressed in E. coli.
SpecificityReactive with 0.2ug of recombinant human P450 1A2. Only slightly crossreacts with recombinant human P450 1A1after prolonged incubation. Does not crossreact with recombinant human cytochromes P450 2E1, 3A4, or 2C10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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