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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B N

The cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid Arachadonic Acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as mNADPH and P-450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). The cytochrome P450 2A subfamily has been divided into two groups: steroid metabolizing and xenobiotic metabolizing enzymes. P450 2A6 belongs to the xenobiotic metabolizing group catalyzing the oxidation of coumarin, cotinine, aflatoxin B1, and other carcinogenic and precarcinogenic drugs.
Catalog #	C9095-15C
P450 enzymes are divided into two groups	steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism. Work in this area is ongoing but studies suggest that all known drug metabolism is mediated by family members: CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1.
Applications	Suitable for use in ELISA, Inhibition Assays. Other applications not tested.
Recommended Dilution	Western Blotting: 1:5000-1:10,000
	Inhibition Assays: 1-20ul
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Serum
Purity	Serum
Immunogen	Purified human CYP2A6 expressed in E. coli.
Specificity	Recognizes human cytochrome p450 2A6 (CYP2A6). This is a neutralizing antibody which is a specific and potent inhibitor of CYP2A6 activity.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.