Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Other applications not tested.

Western Blot: A band is observed at ~48kD in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable. Immunohistochemistry: 1:50-1:200 (ABC, enzymatic detection). Works well with STF fixed tissues that are paraffin embedded. Also works with formalin fixed paraffin embedded tissues but at much lower working dilutions. Recommended counterstain is Gill’s Hematoxylin. Immunocytochemistry: 1:20-1:200 on acetone fixed cells. Optimal dilutions to be determined by researcher.

Adrenal zona glomerulosa of rats on a 7-14 day low sodium diet where the gene is overexpressed.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Rat Aldo-1-MAP aa175-192, KVRQNARGSLTMDVQQSL-MAP.

Supplied as a liquid, 1mg/ml sodium azide, 50% glycerol.

Supernatant.

Recognizes rat cytochrome p450 aldosterone synthase. |Species Crossreactivity: mouse.