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Cytochrome P450 2C19, Recombinant, Human, Microsomal Fraction
Cytochrome P450 2C19, Recombinant, Human, Microsomal Fraction
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| Catalog # | C9095-17L | | Source | Microsomes prepared from insect cells that were infected with a baculovirus containing the cDNA for human CYP2C19 and rabbit cytochrome P450 reductase (CYP rRED). | | Concentration of Cytochrome P450 | 1.21nmol/ml | | Specific Content of Cytochrome P450 | 139 pmol spectral P450/mg of protein. | | Cytochrome P450 Reductase Activity | 0.61 umol of cytochrome c reduced per minute per milligram of protein. | | Mephenytoin Hydrolase Activity | 693 pmol/min/mg of protein. Mephenytoin hydroxylation reactions were performed for 15 minutes @ 37°C in 0.1M potassium phosphate in the presence of 0.3mM S-(+)-mephentoin and 2mM NADPH. The reaction was stopped by the addition of 0.5 volumes of methanol containing 0.2mM phenobarbital (internal standard). The mixture was extracted with methylene chloride. An aliquot of the organic phase was transferred to a fresh tube, and dried down @ 60°C. Analytes were dissolved by addition of 100ul of methanol. | | HPLC conditions: 10ul of sample was injected on a 4.5mm x 70mm 3 um C18 HPLC column. The mobile phase was 18% acetonitrile for 12 minutes with a 97.5% methanol wash for 3 minutes followed by an equilibration step of 18% acetonitrile for 3 minutes. Flow rate was 1.2ml per minute with detection at 204nm. The amount of 4-hydroxymephenytoin was calculated by comparison to a standard curve of authentic 4-hydroxymephenytoin. | | Storage and Stability | Store at -80°C. Avoid repeated freeze/thaw cycles. | | Concentration | ~10mg/ml | | Form | Supplied in 100mM potassium phosphate, pH 7.4, 20% glycerol, 0.1mM EDTA, 1mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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