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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Cytochrome P450 2C9,2C10

Anti -Cytochrome P450 2C9,2C10

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B
Catalog #C9095-17P
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Yields a positive result with 0.1ug of recombinant human cytochrome P450 2C9(2C10) using alkaline phosphatase conjugated anti-rabbit IgG secondary antibody according to the manufacturer’s instructions.
Optimum dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 20mM phosphate buffer, pH 8.0
PurityPurified by ammonium sulfate fractionation. 85% (assessed by visual inspection using Cooomassie blue stained SDS-PAGE gel)
ImmunogenRecombinant human P450 2C9(2C10) expressed in E. coli.
SpecificityRecognizes P450 2C9(2C10) at ~56kD in human liver microsomes. Does not cross-react with recombinant human cytochromes P450 1A1, 1A2, 3A4 or 2E1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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