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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified B

Catalog #	C9095-17P
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000. Yields a positive result with 0.1ug of recombinant human cytochrome P450 2C9(2C10) using alkaline phosphatase conjugated anti-rabbit IgG secondary antibody according to the manufacturer’s instructions.
	Optimum dilutions to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	1mg/ml
Form	Supplied as a liquid in 20mM phosphate buffer, pH 8.0
Purity	Purified by ammonium sulfate fractionation. 85% (assessed by visual inspection using Cooomassie blue stained SDS-PAGE gel)
Immunogen	Recombinant human P450 2C9(2C10) expressed in E. coli.
Specificity	Recognizes P450 2C9(2C10) at ~56kD in human liver microsomes. Does not cross-react with recombinant human cytochromes P450 1A1, 1A2, 3A4 or 2E1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.