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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B

Phase 1 drug metabolism is catalyzed by two groups of monooxygenases, the cytochrome P450s (CYPs) and the flavin monooxygenases (FMOs). Both CYPs and FMOs are localized in the endoplasmic reticulum. Collectively, these enzymes catalyze the oxidation of a very broad range of substrates. Both classes are dependent on NADPH as a source of electrons, but the two classes exhibit significant differences in mechanism and substrate specificity.
Catalog #	C9095-17X
Applications	Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions	Western Blot: 1:1000. Will yield a 2E1 band with 10ug of pyridine-induced rat liver microsomes.
	Optimal dilutions to be determined by researcher.
Please Note	Due to the high sensitivity of the ECL method, very low concentrations of Antigen (2.5ug) are sufficient. Upon prolonged exposures slight crossreactivity may be seen with rat liver microsomes induced with other chemicals.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG
Clone No	4i68
Host	Mouse
Source	Rat
Concentration	Not Determined
Form	Supplied as liquid in TBS.
Purity	Purified
Immunogen	Recombinant rat Cytochrome P450 (2E1). Mol. Wt.: 58.6kD.
Specificity	Recognizes rat Cytochrome P450 2E1. Identification of the polycyclic aromatic induced 2E1 by Western Blots.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.