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You are here:Home » Cloning » Cloning-Primers » Cytochrome P450 4A1, Rat, primers, 902bp amplimer

Cytochrome P450 4A1, Rat, primers, 902bp amplimer

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Specifications

PCR primers for use in the detection and analysis of rat mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 902bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in 50ul sterile water) is sufficient for at least 100 reactions.
Catalog #C9095-18T
C9095-18T1 Upstream Primer 1x50ul (100rxns) Supplied as a liquid in sterile ddH2O.
C9095-18T2: Downstream Primer 1x50ul (100rxns) Supplied as a liquid in sterile ddH2O.
Optimal PCR Conditions Number of cycles 35
Denaturation temperature: 94°C for 1 minute
Annealing temperature: 55°C for 1 minute
Extension temperature: 72°C for 1 minute
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration1uM/ul
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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