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You are here:Home » Cloning » Cloning-Primers » Cytochrome P450 4A2, Rat, primers, 302bp amplimer

Cytochrome P450 4A2, Rat, primers, 302bp amplimer

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Specifications

PCR primers for use in the detection and analysis of rat mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 302bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.
Catalog #C9095-18V
C9095-18V1 Upstream Primer 1x50ul (100rxns). Supplied as a liquid in sterile ddH2O.
C9095-18V2: Downstream Primer 1x50ul (100rxns). Supplied as a liquid in steriler ddH2O.
Optimal PCR ConditionsNumber of cycles: 35
Denaturation temperature: 94°C for 1 min.
Annealing temperature: 55°C for 1 min.
Extension temperature: 72°C for 1 min.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration1uM/ul
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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