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You are here:Home » Molecular Biology » MB-Cloning-Primers » Cytochrome P450 4A3, Rat, primers, 302bp amplimer

Cytochrome P450 4A3, Rat, primers, 302bp amplimer


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PCR primers for use in the detection and analysis of rat mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 302bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.
Catalog #C9095-18X
C9095-18X1 Upstream Primer 1x50ul (1x100rxns). Supplied as a liquid in ddH2O.
C9095-18X2: Downstream Primer 1x50ul (1x100rxns). Supplied as a liquid in ddH2O.
Optimal PCR ConditionsNumber of cycles: 35
Denaturation temperature: 94°C for 1 min.
Annealing temperature: 55°C for 1 min.
Extension temperature: 72°C for 1 min.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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