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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

NADPH-cytochrome P450 reductase is a microsomal component that is essential for transferring reducing equivalents from NADPH to the microsomal heme oxygenase isozymes, HO-1 and HO-2, to the various cytochrome P450 isozymes and to cytochrome c and b5. The reductase is inducible in the liver, phenobarbital and 3-naphthoflavone are good inducers of the enzyme. The rat enzyme has an apparent molecular weight of 78kD and contains both FAD and FMN. Nitric oxide synthase is the other known enzyme that also contains FAD and FMN. The reductase has been isolated from different species and a close range of apparent molecular weights of 76 to 81kD. The amino acid sequence of the reductase from several species has been deduced from cDNA. The enzyme has a short hydrophobic N-terminus that serves to anchor the reductase to the endoplasmic reticulum and nuclear envelope; the reductase without the hydrophobic portion retains its ability to transfer electrons to cytochrome c, but not to cytochrome P450. The 70kD hydrophilic region contains the flavin and NADPH binding domains. The purified enzyme in the presence of a detergent, such as Triton X-100 (0.02%), can reconstitute heme degradation activity of purified or expressed heme oxygenase isozymes(1,3–8).
Catalog #	C9095-24
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot (Colorimetric): 1:2000
	Optimal dilutions to be determined by researcher.
Positive Controls	Rat Liver NADPH Cytochrome P450 Reductase Protein (C9095-23)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid in 0.09% sodium azide, before the addition of glycerol to 40%.
Purity	Serum
Immunogen	Purified rat liver NADPH-Cytochrome P450 reductase.
Specificity	Recognizes an ~78kD protein, corresponding to the apparent molecular mass of rat NADPH Cytochrome P450 Reductase Species Crossreactivity: human, monkey, mouse, bovine, canine, guinea pig, hamster, porcine, rabbit and sheep. Due to the highly conserved nature of this protein we expect that this antibody would have a wide species cross reactivity.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.