| Cytochrome P450 (P450 or CYP) enzymes, a superfamily of b-type heme-containing proteins found in organisms from all |
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| domains of life, are major catalysts in the oxidative |
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| transformation of a diversity of endogenous and exogenous |
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| compounds. CYP26A1 (Cytochrome P450, family 26, subfamily A, polypeptide 1) is a 497aa enzyme in mouse, rat and human (chr10q23). This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in the regulation of gene expression in both embryonic and adult tissues. Highest levels of expression are noticed in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain. |
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| Catalog # | C9095-29 |
| Applications | Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. |
| Recommended Dilution | ELISA: 1:100,000 using 50-100ng control peptide per well |
| Western Blot: 1-10ug/ml using ECL technique |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | C9095-29A-Cytochrome P450 26A1, Human, Control Peptide (CYP26A1) |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 40% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | 15 amino acid synthetic peptide located at the N-terminus of human CYP26A1 (KLH). Species Sequence Homology: Mouse, rat - 100%; chicken - 73%. |
| Specificity | Recognizes human CYP26A1. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
