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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin

Anti -Cytokeratin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse IH
Cellular Localization: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to immunostaining.
Catalog #C9097-01N
ApplicationsSuitable for use in Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8J1147
HostMouse
SourceHuman
ConcentrationAs reported
FormPBS with sodium azide
PurityAs reported
ImmunogenBALB/c mice were immunized with solublized keratin extract. Epitope: from human stratum corneum.
SpecificityThis antibody recognizes keratin polypeptide of 68, 58, 56.5 and 50kD in extracts of stratum corneum.The antibody reacts with squamous, ductal and other complex epithelia.It stains adenocarcinomas, breast, pancreas, bile duct, salivary gland and transit Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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