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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified IH

Cytokeratin filaments consist of a complex family of ~20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells.
Catalog #	C9097-01S
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (Frozen, paraffin): 1:75-1:200 for paraffin. Incubate 30-60 minutes at RT. Requires Proteolytic enzymatic pretreatment.
	Optimal dilutions to be determined by the researcher.
Positive Control	Skin
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Bovine
Concentration	Not Determined
Form	Supplied as a liquid in PBS, pH 7.5, 15mM sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cytokeratin isolated from bovine muzzle epidermis. Cellular Localization: Cytoplasmic.
Specificity	Recognizes bovine cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68kD. Recognizes a broad spectrum of human keratins. Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.