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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin (FITC)

Anti -Cytokeratin (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B FC IF
The C-11 antibody is a broad spectrum antibody which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. This antibody can be applied to methanol- or acetone-fixed frozen sections, and to formalin-fixed, paraffin-embedded human tissues. Increased staining intensity is seen following proteolytic treatment of tested tissue (protease unmasking). Similarly embedded methacarn-fixed material is also suitable for cytokeratin demonstration. This antibody may be used for the localization of cytokeratins using various immunochemical assays including direct immunofluorescence using frozen or fixed tissue sections or cultured cells. It may also be used in flow cytometry of normal and neoplastic epithelial cells. Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells.
Catalog #C9097-01T
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:50
Flow Cytometry: 1:500
Optimal dilutions to be determined by the researcher.
Positive ControlPtk2 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J1145
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.4, 15mM sodium azide. Labeled with FITC.
PurityPurified by Protein A affinity chromatography.
ImmunogenKeratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. Cellular Localization: Cyoplasmic
SpecificityRecognizes human cytokeratins 4, 5, 6, 8, 10, 13 and 18 in Western Blot. Species Crossreactivity: Xenopus laevis, bovine, rat, frog
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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