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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin, Basal Cell

Anti -Cytokeratin, Basal Cell

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Catalog #C9097-03B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:1000
Immunohistochemistry (frozen tissue sections): 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol , aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1113
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in buffer with 0.09% sodium azide.
PurityPurified by Protein G chromatography.
ImmunogenHuman cytokeratins.
SpecificityBroadly reacting cytokeratin antibody specific for cytokeratin 5. Stains basal cells in combined and stratified epithelial tissues. Recognizes the stem cell population, including the amplifying cell in the prostate epithelium. Species crossreactivity: human, rat, rabbit, hamster, canine, chicken, quail and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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