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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH.
Catalog #	C9097-04
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: Formalin fixed sections.
	Note: Pretreatment with type XXIV protease (0.5mg/ml) produces best results. HIER (Microwave citrate buffer pH 6.0) produces some staining.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q1048
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide
Purity	Purified by Protein A affinity chromatography.
Immunogen	Keratin 1 and 10
Specificity	Recognizes the suprabasal cells of human stratified squamous epithelia. Species Crossreactivity: mouse and porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.