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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 10

Anti -Cytokeratin 10

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
AMH is expressed post-natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth. AMH is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulfide linked precursor that is cleaved to release the mature 30kD homodimer.
Catalog #C9097-09D
ApplicationsSuitable for use in Immunohistochemistry (frozen, paraffin). Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50, requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.
HybridomaSpleen cells from immunized mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.
Recommended Positive Control TissueHuman normal skin
Recommended Secondary ReagentsF(ab')2 rabbit anti mouse IgG:HRP
Recommended Negative ControlsMouse IgG1 for use in human studies.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A400
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, .0.09% sodium azide.
PurityTissue Culture Supernatant
ImmunogenCytoskeletal extract from epidermis of normal human skin.
SpecificityRecognizes the 56kD cytokeratin 10, found in a variable number of cells in the suprabasal layer of stratified epithelia, and in more differentiated areas of some squamous carcinomas.
Species CrossreactivityHuman, porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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