| AMH is expressed post-natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth. AMH is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulfide linked precursor that is cleaved to release the mature 30kD homodimer. |
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| Catalog # | C9097-09D |
| Applications | Suitable for use in Immunohistochemistry (frozen, paraffin). Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 1:50, requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose. |
| Hybridoma | Spleen cells from immunized mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line. |
| Recommended Positive Control Tissue | Human normal skin |
| Recommended Secondary Reagents | F(ab')2 rabbit anti mouse IgG:HRP |
| Recommended Negative Controls | Mouse IgG1 for use in human studies. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 6A400 |
| Host | Mouse |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid, .0.09% sodium azide. |
| Purity | Tissue Culture Supernatant |
| Immunogen | Cytoskeletal extract from epidermis of normal human skin. |
| Specificity | Recognizes the 56kD cytokeratin 10, found in a variable number of cells in the suprabasal layer of stratified epithelia, and in more differentiated areas of some squamous carcinomas. |
| Species Crossreactivity | Human, porcine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
