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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 10+13

Anti -Cytokeratin 10+13

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IC FC
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9 ? 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Catalog #C9097-09J
Cellular Localization Cytokeratin 10 is present in keratinising stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomasCytokeratin 13 is present in non-cornified squamous epithelia except cornea and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia as well as carcinomas derived from these tissues.
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry (Fr) (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No8J1169
HostMouse
SourceHuman
ConcentrationNot determined
PurityTissue culture supernatant
ImmunogenCytoskeletal preparation extracted from human ectocervical epithelium.
SpecificityThis antibody reacts with cytokeratin 10 and 13 in fixed cells and frozen tissue, but exclusively with cytokeratin 13 in formalin fixed paraffin embedded tissue. Species Crossreactivity: Crossreacts with Human and Cat. Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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