| Cytokeratin 10 (CK10) is a heterotetramer of two type I and two type II keratins. CK10 is typically associated with cytokeratin 1 (CK1). CK10 is expressed in all suprabasal cell layers and it is part of acidic/high molecular weight keratin subfamily. Defects in CK10 causes Epidermolytic hyperkeratosis (EHK), a hereditary skin hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum (1). CK10 has also been linked to cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. CK10, along with CK14, 15, 16, and 20, can be used to determine if a neoplasm originated from an epithelial or non-epithelia origin, such as the determination of metastatic malignant tumors of epithelial origin (2). |
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| Catalog # | C9097-11G |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:50,000-100,000 |
| Immunohistochemistry: 1:100-250 |
| Immunocytochemistry: 1:100-250 |
| Immunoprecipitation: 1:30 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9E243 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues in the C-term of human Cytokeratin 10. |
| Specificity | Recognizes human Cytokeratin 10. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
