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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Catalog #	C9097-12C
Applications	Suitable for use in Immunofluorescence, Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material; protease pretreatment improves the performance of this excellent marker) and Immunoblot.
Recommended Dilution	Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F225
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cytokeratin 13 of Mr 54,000 purified from human esophagus.
Specificity	Antigen Recognized in Human, bovine and rat. Reactivities on Cultured Cell lines from squamous cell CA. Cell Lines (tested so far) e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder. Represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.