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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin 13

Anti -Cytokeratin 13


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Catalog #C9097-12C
ApplicationsSuitable for use in Immunofluorescence, Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material; protease pretreatment improves the performance of this excellent marker) and Immunoblot.
Recommended DilutionImmunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5F225
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
PurityPurified by Protein A affinity chromatography.
ImmunogenCytokeratin 13 of Mr 54,000 purified from human esophagus.
SpecificityAntigen Recognized in Human, bovine and rat. Reactivities on Cultured Cell lines from squamous cell CA. Cell Lines (tested so far) e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder. Represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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