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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IC FC

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium.
Catalog #	C9097-13D
Applications	Suitable for use in Immunohistochemistry (frozen sections), Immunocytochemistry, Flow Cytometry, and Western Blotting. Other applications not tested.
	Optimum dilutions to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	4A30
Host	Mouse
Source	Human
Concentration	1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Cytokeratin 14
Specificity	Reacts with basal cell compartments of stratified and combined epithelia. Species crossreactivity: Human, rat, canine, porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.