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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 14 (FITC)

Anti -Cytokeratin 14 (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH FC
Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types.Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.
Catalog #C9097-13T
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: Use 10ul to label 10e6 cells.
Immunohistochemistry (Frozen/Paraffin): For paraffin sections, microwave treatment or high temperature antigen unmasking technique is recommended. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended is neutral buffered formalin.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG3
Clone No8J1174
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 1% BSA.,10mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
PurityPurified
ImmunogenSynthetic peptide corresponding to the extreme carboxy terminal of human keratin 14 (last 15 residues). (Thyroglobulin). Cellular Localization: Cytoplasmic.
SpecificityRecognizes human cytokeratin 14.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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