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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified B IH

Cytokeratin 14 is a type I intermediate filament. It is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.
Catalog #	C9097-14B
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: Suitable for staining formalin fixed wax sections or frozen tissues. Recommended to either microwave treat fixed tissue sections or high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use is neutral buffered formal saline
	Optimal dilutions to be determined by researcher.
Hybridoma	NS1 myeloma cells with spleen cells from Balb/c mice.
Source	Tissue culture supernatant
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	2Q1030
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as liquid in PBS, 10mM sodium azide, 1mg/ml BSA.
Purity	Purity by ammonium sulfate precipitation followed by ion exchange chromatography.
Immunogen	Synthetic peptide corresponding to the last 15 residues of extreme C-terminus of human keratin 14 (Thyroglobulin coupled).
Specificity	Recognizes human Cytokeratin 14.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.