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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 18 (Biotin)

Anti -Cytokeratin 18 (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IH IC
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70kD and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Catalog #C9097-19V
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500. Predicted molecular weight 45kD.
Immunohistochemistry (paraffin-embedded sections): 1:10-1:100.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J1191
HostMouse
SourceHuman
Concentration~1mg/ml
Form15mM Sodium Azide. PBS. pH 7.4. Labeled with Biotin.
PurityPurified
ImmunogenHuman breast carcinoma cell line PMC-42.
SpecificityRecognizes human Cytokeratin peptide 18. Does not react with a wide range of other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCK 18, CYK18, Cytokeratin endo B, Keratin 18, Keratin D, Keratin type I cytoskeletal 18, Kerd, KRT18


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