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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH IC FC

Cytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3).
Catalog #	C9097-20B
Applications	Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:15
	Western Blot: 1:50,000
	Immunoprecipitation: 1:100
	Immunohistochemistry: 1:250-1:500
	Immunocytochemistry: 1:250-1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA, 40% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to residues on the C-terminus of human CK-18.
Specificity	Recognizes human Cytokeratin 18.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	CK 18, CK18, CYK18, Cytokeratin endo B, K18, Keratin 18, Keratin D, Keratin type I cytoskeletal 18, Kerd, KRT18