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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 18

Anti -Cytokeratin 18

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum B IH IC
Represents an excellent marker for all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Catalog #C9097-20G
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:2000 (ECL).
Immunohistochemistry (Frozen): 1:200; Incubate 1 hr at RT.
Immunohistochemistry (Paraffin-embedded tissue): 1:100
Incubate > 1 hr at RT; extended time for paraffin sections.
Optimal dilution determined by the researcher.
Positive ControlsTumors Specifically Detected: All adeno-Ca tested, undifferentiated CA, hepatocellular CA, cervix Ca.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.5% BSA, 0.09% sodium azide.
PuritySerum
ImmunogenHuman cytokeratin 18, purified from MCF-7.
SpecificityRecognizes acidic human cytokeratin 18 (Mr 45,500). No crossreaction with other keratins and intermediate filament proteins. Species Crossreactivity: mouse and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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