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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 18

Anti -Cytokeratin 18

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IF
Catalog #C9097-21A1
ApplicationsSuitable for use in Immunoblot, Immunofluorescence and Immunohistochemistry (frozen sections). Useful for the discrimination of adenocarcinomas and mesotheliomas from squamous cell carcinoma and non-epithelial tumors. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:5-1:10
Positive ControlAdenocarcinoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F301
HostMouse
ConcentrationNot determined
FormSupplied as a liquid with 0.1% sodium azide.
PurityCulture supernatant
Immunogen45kD cytokeratin 18, isolated from HeLa cells.
SpecificityRecognizes Keratin 18. Specific to simple and glandular epithelial cells from the gastrointestinal tract, the respiratory tract and the urogenital tract, as well as endocrine and exocrine tissues and myoepithelial cells. No reaction with stratified squamous epithelia. In the transitional epithelium of the bladder this clone reacts only with superficial (umbrella) cells.
Species CrossreactivityHuman, most mammals including mouse, rat, rabbit, canine, porcine; birds (chicken).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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