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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 18

Anti -Cytokeratin 18

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Catalog #C9097-21D
ApplicationsSuitable for use in Immunohistochemistry (frozen tissue, cytological material), Immunoblot (Western) and ELISA.
Recommended DilutionImmunohistochemistry: 1:10; Incubation Time 1 hr at RT.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F228
HostMouse
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA and 0.09% sodium azide. Reconstitute to a final volume of 1ml.
PurityPurified by Protein A affinity chromatography.
ImmunogenCytoskeletal preparation from tumor cell line MCF-7.
SpecificityAntigen Recognized in Human, bovine; negative with mouse In Species (tested so far).
Tumors Specifically DetectedAll adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA Reactivities on Cultured MCF-7, HeLa. A-431, PLC, RT 112. Represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (cytokeratin 18) of all simple type and complex epithelia.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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