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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 18

Anti -Cytokeratin 18

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #C9097-23D1
Represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
ApplicationsSuitable foruse in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:20; Incubation Time 1h at 37°C, extended with paraffin sections microwave treatment is recommended (overnight at 4C).
Positive ControlsHeLa and MCF-7.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol , aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F227
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA and 0.09% sodium azide. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cytokeratin 18 from HeLa cytoskeletal preparation.
SpecificityRecognizes human Cytokeratin 18. Species Crossreactivity: mouse, rat, bovine, porcine, canine, hamster, sheep, fish (trout).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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