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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 18 (Biotin)

Anti -Cytokeratin 18 (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Catalog #C9097-23D2
ApplicationsSuitable for use in ELISA, Immunoblot and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material).
Recommended DilutionImmunohistochemistry: 1:10; Incubation Time 1hr at 37°C.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F227
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4. Conjugated to Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cytokeratin 18 from HeLa cytoskeletal preparation.
SpecificitySpecies Crossreactivity: Human, mouse, rat, bovine, porcine, canine, hamster, sheep, fish (trout)
Reactivities on Cultured HeLa and MCF-7. Represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detectedAll adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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