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C9097-23H Mouse Anti-Cytokeratin 18

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05783
Isotype
IgG1
Clone Number
RCK106
Grade
Purified
Applications
FC IC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

Applications
Suitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cytoskeletal preparation of human bladder carcinoma cell line T24
Form
Supplied as a liquid in 0.09% sodium azide.
Purity
Purified IgG from culture supernatant.
Specificity
Recognizes human Cytokeratin 18. Reacts exclusively with human glandular epithelial cells of the digestive, respiratory and urogenital tracts, endocrine and exocrine cells and mesothelial cells as well as adenocarcinomas originating from them. Recognizes human only. Does not react with other species.
References
1. Smedts, F. et al. Keratin expression in cervical cancer. Am. J. Path. (1992) 141:497–511. 2. Raats, JMH. et al. Assembly of amino-terminal deleted desmin and vimentinfree cells. J. Cell Biol. (1990) 111:1971–1985. 3. Ramaekers, F. et al. Use of monoclonal antibodies to keratin 7 in a differential diagnosis of adenocarcinomas. Am. J. Pathol. (1990) : 4. Schaafsma, HE. et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am. J. Path. (1990) 136:329–343
USBio References
No references available
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