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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Cytokeratin 18, Caspase-cleaved

Anti -Cytokeratin 18, Caspase-cleaved

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH IC FC
Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder and trophoblasts. Cytokeratin 18, Caspase-cleaved monoclonal antibody has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site (M30-neoepitope) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells.
Catalog #C9097-23W
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry
Immunohistochemistry and Western Blot. Other applicatiions not tested.
Recommended DilutionsFlow Cytometry: 1:100
Immunocytochemistry: 1:100
Immunohistochemistry (Frozen, Paraffin): 1:100.
Western Blot: 1:100.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6D45
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, PEG, sucrose, 0.09% sodium azide.
PurityPurified
ImmunogenCK18 (cytokeratin 18) fragments purified from supernatant from human carcinoma cell line WiDr CCL218
SpecificityRecognizes the human CK18-Asp396 neo-epitope (M30) of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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