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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Cytokeratin 18, Caspase-cleaved (Biotin)

Anti -Cytokeratin 18, Caspase-cleaved (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH IC FC
Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder and trophoblasts. Cytokeratin 18, Caspase-cleaved monoclonal antibody has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site (M30-neoepitope) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells.
Catalog #C9097-23W1
Applications
Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:100
Immunocytochemistry: 1:100
Immunohistochemistry (Paraffin and Frozen): 1:100
Western Blot: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6D45
HostMouse
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, PEG, sucrose and 0.09% sodium azide. Labeled with biotin.
PurityPurified supernatant
ImmunogenCK18 (cytokeratin 18) fragments purified from supernatant from human carcinoma cell line WiDr CCL218.
SpecificityRecognizes the human CK18-Asp396 neo-epitope of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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