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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Cytokeratin 18, Caspase-cleaved (FITC)

Anti -Cytokeratin 18, Caspase-cleaved (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IC FC
Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder, trophoblasts. Cytokeratin 18, Caspase-cleaved monoclonal antibody has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site (M30-neoepitope) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells.
Catalog #C9097-23W2
ApplicationsSuitable for use in Immunocytochemistry and Flow Cytometry. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1:100
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6D45
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS containing 0.1% BSA, PEG, sucrose and 0.09% sodium azide.
PurityPurified supernatant
ImmunogenCK18 (cytokeratin 18) fragments purified from supernatant from human carcinoma cell line WiDr CCL218.
SpecificityRecognizes the human CK18-Asp396 neo-epitope of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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