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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 19

Anti -Cytokeratin 19

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium. Cytokeratin 19 is the smallest human cytokeratin (40kD) and is found in many simple and invariably in some non-keratinizing stratified squamous epithelia. All adenocarcinomas tested have been positive for cytokeratin 19.
Catalog #C9097-24A
SourceCell culture
ApplicationsSuitable for use in Indirect Immunoperoxidase, Western Blot, Immunohistochemistry (frozen, Paraffin 1:50-1:100). Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Indirect Immunoperoxidase): 1:50. Paraf n embedded human epithelial tissue sections. Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Optimal dilutions to be determined by the researcher.
Positive Control Normal breast tissue
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3C99
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1%, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenMammary organoids
SpecificityReacts with the smallest human cytokeratin (Mr 40kD). No crossreactivity with other cytokeratins. Reacts strongly with luminal (secretory) epithelial cells but does not stain the basal (myoepithelial) cells. There is a subset ofsecretory epithelial cells which were Keratin 19 and are thought to be precursors to keratin 19+ cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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