Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 19

Anti -Cytokeratin 19

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH IC FC
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Catalog #C9097-24B
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:100-1:1000
Immunohistochemistry (Frozen, paraffin): 1:100-1:200 using avidin-biotinylated HRP complex (ABC) for detection. Paraffin tissues require pretreatment with pepsin or trypsin.
Flow Cytometry: 1:100-1:200
Optimum dilutions to be determined by researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No4A36
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
PurityPurified
ImmunogenCytoskeletal preparation of the human bladder carcinoma cell line T24.
SpecificityRecognizes cytokeratin 19 from glandular-type epithelia and most carcinomas. Does not recognize cytokeratin from hepatocytes and hepatocellular carcinoma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links