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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IH IC FC

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Catalog #	C9097-24B
Applications	Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:100-1:1000
	Immunohistochemistry (Frozen, paraffin): 1:100-1:200 using avidin-biotinylated HRP complex (ABC) for detection. Paraffin tissues require pretreatment with pepsin or trypsin.
	Flow Cytometry: 1:100-1:200
	Optimum dilutions to be determined by researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4A36
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Purity	Purified
Immunogen	Cytoskeletal preparation of the human bladder carcinoma cell line T24.
Specificity	Recognizes cytokeratin 19 from glandular-type epithelia and most carcinomas. Does not recognize cytokeratin from hepatocytes and hepatocellular carcinoma.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.