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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 19

Anti -Cytokeratin 19

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IF
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium. Cytokeratin 19 is the smallest human cytokeratin (40kD) and is found in many simple and invariably in some non-keratinizing stratified squamous epithelia. All adenocarcinomas tested have been positive for cytokeratin 19.
Catalog #C9097-24B1
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (ABC): 1:10-1:20 on paraffin and frozen sections. Dilute immediately before use with PBS or TBS. No proteolytic pretreatment required. HEIR in citrate buffer is useful.
Optimal dilutions to be determined by the researcher.
Positive ControlGlandular epithelia.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F299
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.1% sodium azide.
PuritySupernatant
ImmunogenHuman bladder cell line T24.
SpecificityRecognizes human Cytokeratin K19 found in most glandular simple type epithelia, adenocarcinoma, and most squamous cell carcinoma at 40kD. Species Crossreactivity: Most mammals. Does not crossreact with mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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