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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified IH IF

This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7
Catalog #	C9097-24G
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence : 1:50-1:100
	Immunohistochemistry (paraffin-embedded sections): 1:500 Staining of formalin-fixed tissues requires digesting tissue sections with pepsin for 30 min at 37ºC. Optimal dilutions to be determined by the researcher.
Recommended Positive Control	Skin
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, BSA and 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide derived from the C-terminal region of human cytokeratin 19. Cellular Localization: Cytoplasmic.
Specificity	Recognizes human Cytokeratin 19. Species Crossreactivity: mouse. Other species have not been tested.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	ck19, Cytokeratin 19, k19, k1cs, Keratin 19, Keratin type i 40kD, krt19, mgc15366