| This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7. |
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| Catalog # | C9097-24N |
| Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:500 |
| Western Blot: 1:500 |
| Immunoprecipitation: 1:500 |
| Immunohistochemistry (Paraffin): 1:500 |
| Immunocytochemistry: 1:500 |
| Immunofluorescence: 1:500 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 8J1200 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Biotin. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Human mammary carcinoma cell line MCF-7. |
| Specificity | Recognizes rod domain of cytokeratin peptide 19 (~40kD) in human tissue. Cytokeratin 19 is not expressed in hepatocytes. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | ck19, Cytokeratin 19, k19, k1cs, Keratin 19, Keratin type i 40kD, krt19, mgc15366 |
