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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH IF

Catalog #	C9097-27A
Tumors specifically detected	All tested adenocarcinoma; cholangio ca. of liver; renal cell carcinoma; transitional cell ca. of the bladder; ovary ca.; squamous cell ca. of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast ca.; thymoma. Represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.
Applications	Suitable for use in ELISA, Immunoblot, Immunofluorescence microscopy, Immunohistochemistry (frozen tissue, paraffin-embedded tissue (microwave procedure highly recommended).
Recommended Dilution	Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 h at RT, extended with paraffin.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F229
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cytokeratin 19 of Mr 40,000; from cultured human MCF-7 cells
Specificity	Antigen Recognized in Human. Reactivities on Cultured MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Polypeptide reacting: Mr 40 000 polypeptide (cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa311-335 (QSQLSMKAALEDTLAETEARFGAQL) of the a-helical fragment.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.