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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 19

Anti -Cytokeratin 19

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IF
Catalog #C9097-27A
Tumors specifically detected All tested adenocarcinoma; cholangio ca. of liver; renal cell carcinoma; transitional cell ca. of the bladder; ovary ca.; squamous cell ca. of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast ca.; thymoma. Represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.
ApplicationsSuitable for use in ELISA, Immunoblot, Immunofluorescence microscopy, Immunohistochemistry (frozen tissue, paraffin-embedded tissue (microwave procedure highly recommended).
Recommended DilutionImmunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 h at RT, extended with paraffin.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F229
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
PurityPurified by Protein A affinity chromatography.
ImmunogenCytokeratin 19 of Mr 40,000; from cultured human MCF-7 cells
SpecificityAntigen Recognized in Human. Reactivities on Cultured MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Polypeptide reacting: Mr 40 000 polypeptide (cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa311-335 (QSQLSMKAALEDTLAETEARFGAQL) of the a-helical fragment.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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