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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 19

Anti -Cytokeratin 19

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC
Cytokeratin polypeptide 19 (CK19) is a type I intermediate filament protein that is expressed in stratified and simple-type epithelia (1). Cytokeratins 8 and 19 concentrate at costameres of striated muscle and copurify with the dystrophin-glycoprotein complex, perhaps through
the interaction of the cytokeratins with the actin-binding domain of dystrophin. Studies in COS-7 cells and in vitro showed that dystrophin binds directly and specifically to K19. Results show that dystrophin interacts through its actin-binding domain with CK19 specifically and are consistent
with the idea that cytokeratins associate with dystrophin at the sarcolemma of striated muscle (2). CK19 is synthesized mainly in embryonic and adult simple epithelia, but has also been found in stratified epithelia as well. CK19 is the smallest known keratin and is remarkable in that, contrary to all other keratins, it does not have a designated partner for the formation of filaments, implying that regulation of its expression is different from other keratin-encoding genes (3).
Catalog #C9097-27G
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:10,000-50,000
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Flow Cytometry: 1:20
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E247
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human CK-19.
SpecificityRecognizes human Cytokeratin 19.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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