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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH IC

Catalog #	C9097-28
Tumors Specifically Detected	All tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma and thymoma. Represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.
Applications	Suitable for ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Frozen): 1:10 with PBS, pH 7.4. Incubate for 1 hr at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	5F230
Host	Mouse
Source	Human
Concentration	~0.05mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cytokeratin 19 from MCF-7 cells. Epitope: aa352-368 (VRADSERQNQEYQRLMD) of the a-helical fragment.
Specificity	Recognizes human Cytokeratin 19 at ~40kD on human glandular epithelia. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.