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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IC FC

Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Catalog #	C9097-29A
Applications	Suitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Frozen, Paraffin): 1:10-1:20 in PBS, pH 7.4. Incubate for 1 hour at RT or overnight at 2-8ºC. Microwave treatment required for paraffin sections.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F216
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC).
Purity	Purified by affinity chromatography.
Immunogen	Electrophoretically purified Cytokeratin (CK) 20 from human intestinal mucosa.
Specificity	Recognizes human Cytokeratin 20, Mr 46kD. Reactivities on Cultured Cell Lines
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.