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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 20 (FITC)

Anti -Cytokeratin 20 (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC FC
Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Catalog #C9097-29A
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen, Paraffin): 1:10-1:20 in PBS, pH 7.4. Incubate for 1 hour at RT or overnight at 2-8ºC. Microwave treatment required for paraffin sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F216
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by affinity chromatography.
ImmunogenElectrophoretically purified Cytokeratin (CK) 20 from human intestinal mucosa.
SpecificityRecognizes human Cytokeratin 20, Mr 46kD. Reactivities on Cultured Cell Lines
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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