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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Catalog #	C9097-29C
Applications	Suitable for use in Immunohistochemistry and Western Blot.
	Other applications not tested.
Recommended Dilution	Immunohistochemistry (Frozen sections and cytological material): 1:10 in PBS, pH 7.4 Incubation Time: 1 hr at RT or overnight at 4°C.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F218
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA and 0.09% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Electrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa.
Specificity	Recognizes human Cytokeratin 20 at ~46kD. Does not recognize rat. Cell Lines (tested so far) CaCo-2, RT-4. Reactivities on Cultured HT-29, LoVo, DLD-1, SW 1116. Polypeptide recognized: protein IT.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.