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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Catalog #	C9097-29D
Applications	Suitable for use in Immunohistochemistry (paraffin sections after microwave treatment) and Immunoblot (Western).
Recommended Dilution	Immunohistochemistry: 1:10 in PBS (pH 7.4) (1:100 when applying the microwave method); Incubation Time 1 hr at RT or overnight at 2-8°C.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F219
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA and 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Electrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa.
Specificity	Antigen Recognized in Human; not with rat and mouse. Reactivities on Cultured HT-29, LoVo, DLD-1, SW 1116. Polypeptide recognized: protein IT (cytokeratin No. 20; Mr 46,000).
Cell Lines (tested so far) CaCo-2, RT-4.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.